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Classifications

  1. Acad. J. Med. Plant.


ajmp

Research Article

Academia Journal of Medicinal Plants 7(2): 042-054, February 2019
DOI: 10.15413/ajmp.2018.0200
ISSN: 2315-7720
İ2019 Academia Publishing

Abstract


Extracts from Syzygium guineense inhibit cervical cancer cells proliferation through cell cycle arrest and triggering apoptosis

Accepted 3rd January, 2019

 

Tankeu Nzufo Francine1,2,4*, Pieme Constant Anatole1, Jyoti Kode2,3, Trupti Pradhan4, Lenta Ndjakou Bruno5, Ngogang Yonkeu Jeanne3,4 and Shubhada Chiplunkar 3,4

1Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Science, University of Yaoundé I, P. O. Box 1364, Yaoundé, Cameroon.
2Anti-Cancer Drug Screening Facility (ACDSF); Advanced Centre for Treatment, Research and Education in Cancer; Tata Memorial Centre, Navi Mumbai 410210-India.
3Homi Bhabha National Institute (HBNI), Training School Complex, Anushakti Nagar, Mumbai 400085, India.
4Laboratory of Immunology; Advanced Centre for Treatment Research and Education in Cancer; Tata Memorial Centre, Navi Mumbai 410210-India.
5Department of Chemistry, Higher Teacher Training College, University of Yaoundé 1, P.O. Box 47, Yaoundé, Cameroon.
 

Cancer is the second most deadly disease worldwide. However, its therapeutic treatment still faces several impediments mainly related to the severity of side effects and multidrug resistance of current cancer chemotherapeutics. This suggests a permanent need towards searching new compounds which could be less cytotoxic to normal cells and overcome drug resistance. Syzygium guineense is a plant of the Myrtaceae family widely distributed in tropical countries used in traditional Cameroonian medicine for the treatment of liver cirrhosis and vaginal infections. In this study, we investigated the cell growth inhibitory potential of leaf and bark extracts of S. guineense on human cervical cancer cells, SiHa and HeLa, followed by the study of the effects of active fractions on cell cycle as well as their potential to induce apoptosis. The Bax and Bcl-2 protein content were assessed in the most sensitive cell line (SiHa). The GC/MS analysis helped to identify the putative compounds responsible for the anti-proliferative and cytotoxic effects. The results clearly showed that fractions L4 and L5 derived from the ethanolic leaf extracts exerted the most potent anti-proliferative effect in both SiHa and HeLa cells. GI50 values were respectively 48.11 and 33.64 µg/mL in SiHa and 56.43 and 41.69 µg/mL in HeLa. Treatment of cells with L4 and L5 caused prominent S and G2/M phase arrest in SiHa and HeLa, respectively. The characterization of the mechanism underlying the apoptotic effect in SiHa showed an upregulation of Bax and a down regulation of Bcl-2. These results suggest anti-proliferative potential of S. guineense secondary metabolites due to cell cycle arrest and apoptosis induction mediated through Bax-dependent mitochondrial pathway.

Key words: apoptosis, Bax, Bcl-2, cell cycle, SRB assay, Syzygium guineense.
       

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Tankeu NF, Pieme CA, Jyoti K, Trupti P, Lenta NB, Ngogang YJ, Shubhada C (2018). Extracts from Syzygium guineense inhibit cervical cancer cells proliferation through cell cycle arrest and triggering apoptosis. Acad. J. Med. Plants. 7(2): 042-054.

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