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Research Article

Academia Journal of Biotechnology 6(3): 066-073, March 2018
DOI: 10.15413/ajb.2018.0107
ISSN: 2315-7747
2018 Academia Publishing


Synthesis of silver nanoparticles from Centella asiatica(L.) plant and in vitro derived callus culture: Assessment of antibacterial activity

Accepted 20th March, 2018
Sapna Thakur1*, Shweta Thakur2, Mamta Shandilya3 and Radheshyam Rai4

1Akal college of Agriculture, Department of Biotechnology, Eternal University, HP, India.
2Department of Physics, University of Aveiro, 3810-193 Aveiro, Portugal.
3Department of Physics, Shoolini University, HP, India.
4Akal college of Basic Sciences, Department of Physics, Eternal University, HP, India.


Callus raised from the leaf explants of Centella asiatica(L) was evaluated in the present study. The plant is pharmacologically very important and its consumption as underutilized green leafy vegetable affluent in micronutrients is communally conscientious for its threatened status. Therefore, there is an importunate need to preserve its germplasm so that pharmacologically active constituent can be made available all over the year without causing loss of species. Maximum callusing was observed in MS + benzylaminopurine (BAP, 0.5 mg/l) + α-napthalene acetic acid (NAA, 0.3 mg/l) in leaf explants with callus induction frequency of 75%. The callus and leaf parts of in vivo plant were used for qualitative primary metabolites in its methanolic extracts. This study evaluates the significance of antibacterial activity of silver nanoparticles synthesized from plants extracts and in vitro derived callus extracts of C. asiatica(L.) and characterized by UV spectroscopy, FTIR analysis. Antibacterial activity was evaluated using the well diffusion assay, and zone of inhibition of synthesized silver nanoparticles which were tested against the gram-positive bacteria and the gram-negative bacteria.

Key words: Centella asiatica(L), callus culture, primary metabolites, AgNO3, antibacterial activity, AgNPs (silver nanoparticles).


This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Thakur S, Thakur S, Shandilya M, Rai R (2018). Synthesis of silver nanoparticles from Centella asiatica(L.) plant and in vitro derived callus culture: Assessment of antibacterial activity. Acad. J. Biotechnol. 6(3): 066-073.

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