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Research Article

Academia Journal of Biotechnology 6(4): 080-089, April 2018
DOI: 10.15413/ajb.2018.0110
ISSN: 2315-7747
2018 Academia Publishing

Abstract


In-vitro conservation of multipurpose tree species of genus Acacia
 

Accepted 24th April, 2018
 
Rukhsana Kausar1*, Alvina Nazir1 and Hinagul2

1Department of Environmental Sciences, International Islamic University Islamabad, Pakistan.
2Department of Plant Sciences, Quaid e azam University Islamabad, Pakistan.

 

Various species of Acacia are suitable for tree tissue culture due to their established wasteland recoverable ability and ecological significance. The aim of this present study was in-vitro conservation of Acacia nilotica and Acacia modesta species to optimize a protocol by employing tissue culture technique in-vitro regeneration of this plant. Leaves and nodes were used as explants for both species. Results indicates that maximum number of Callus obtained for Acacia modesta at the concentration (4.0 mg/L) of Indole-3- acetic acid and 6-Benzylaminopurine and Acacia nilotica at the concentration (3.0 mg/L) of Naphthalene Acetic Acid and 6-Benzylaminopurine. Maximum shoots were obtained for both species of Acacia at the concentration (2.0 mg/L) of Kinetin. Phytochemical analysis was performed to find out some important secondary metabolites like alkaloids, terpenoids, phenols, phtyosterol, tannins and steroids but only phytosterol and tannins were found in tested plants. The relevance of tissue culture methods has gained momentum to meet the growing demands for biomass and forest products. Furthermore, these plants can be safe from extinction by using tissue techniques as thick forest is economically imperative and trustworthy   for better environment of a country.

Keywords: Acacia nilotica, Acacia modesta, in-vitro multiplication, multipurpose tree, phytochemicals.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Kausar R, Nazir A, Hinagul (2018). In-vitro conservation of multipurpose tree species of genus Acacia. Acad. J. Biotechnol. 6(4): 080-089.

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