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Research Article

Academia Journal of Biotechnology 5(7): 099-103, July 2017
DOI: 10.15413/ajb.2017.0304
ISSN: 2315-7747
2017 Academia Publishing

Abstract


Arcanobacterium pyogenes infected gazelles at King Khalid Wildlife Research Centre, Thumamah, Kingdom of Saudi Arabia
 

Accepted 3rd July, 2017

 
Mohammed Shiekh Alsaggaf1*, Osama Badri Mohammed2 and Omar H.M. Shair3

1Shaqra University, College of Science and Humanitarian Studies, Qwaieah 11971, Saudi Arabia.
2King Saud University Research Chair, Department of Zoology, College of Science, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia.
3King Saud University College of Science, Department of Botany and Microbiology, Saudi Arabia.

 

The genus Archanobacterium was studied according to its cell wall constituents and the building of the respiratory quinines to house bacterial strains associated to the species of Corynebacterium haemolyticum. This research was carried out to identify phenotypic and genotypic properties of Arcanobacterium pyogenes found in infected lungs after death from gazelles kept at Thumamah, Riyadh Saudi Arabia. Out of hundred Gazella gazella 35% were positive and Gazella marica (45%), while 20% of G. gazelle and G. marica were negative. Bacteria from lung lesions of G. gazella and G. marica were isolated using 5% sheep blood agar. It was sub-cultured several times on 5% blood agar plates until single colonies were obtained. Bacterial DNAs were extracted from the single colonies obtained by using Qiagen DNeasy Blood and Tissue Kit (QIAGEN GmbH, Hilden) following the manufacturer’s instructions. The primers used were very specific and gave rise to magnification of approximately 1400 bp. Purified DNA was amplified by using 16S rDNA and beta subunit of RNA polymerase coding gene rpoB of the 10 isolates from species were amplified by the oligonucleotide primers described Khamis and a species precise primers of the 16S-23S rDNA intergenic piece region (ISR). All PCRs were performed using a MBS ThermoHypaid PCR machine. Neighbour joining and maximum likelihood (ML and NJ consensus) tree based on 419 bp fragments of 16S rRNA members’ genes of A. pyogenes species were constructed.

Key words: Arcanobacterium, gazelles, lung, lesions, pyogenes

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Alsaggaf MS, Mohammed OB, Shair OHM (2017). Arcanobacterium pyogenes infected gazelles at King Khalid Wildlife Research Centre, Thumamah, Kingdom of Saudi Arabia. Acad. J. Biotechnol. 5(7): 099-103.

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