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Research Article

Academia Journal of Agricultural Research 5(9): 218-223, September 2017
DOI: 10.15413/ajar.2017.1212
ISSN: 2315-7739
2017 Academia Publishing

Abstract

 

Chlorophyll and morphological mutation induction in soybean (Glycine max (L.) Merrill.) through modified single seed bulk method

Accepted 11th August, 2017
 

Khan MH * and Tyagi SD.

Department of Genetics and Plant Breeding, Kisan (PG), College, Simbhaoli, Hapu India.

 

The present investigation was undertaken to study the frequency and spectrum of chlorophyll and morphological mutants induced by gamma rays, EMS and their combination in soybean (Glycine max (L.) Merrill.). Chlorophyll mutants were recorded as percent M2 seedlings and in both the cultivars four types of chlorophyll mutants’ viz., albina, xantha, chlorina and viridis were observed. The frequency of the chlorophyll mutants increased with the increase in the dose and concentration of the mutagens. Pusa-16 exhibited high chlorophyll mutants as compared to PK-1042 and the frequency of mutants was higher in combined treatment followed by gamma rays and EMS in both the cultivars. In Pusa-16 the frequency of mutants were in the following order: albina > viridis > chlorina > xantha, however, in PK-1042 they were present in the following order: albina > xantha > viridis > chlorine. With regard to the morphological mutants, five types of mutants viz., tall, dwarf, early maturity, gigas and bold seeded were isolated in Pusa-16 and three types of mutants viz., black pod, black spotted seed and early maturing were observed in PK-1042. High frequencies of mutants in Pusa-16 were recorded from combined treatment, while in Pusa-16 highest frequency was observed in EMS treatment.

Key words: Gamma rays, EMS, chlorophyll mutants, morphological mutants, soybean
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:

Khan MH, Tyagi SD (2017). Chlorophyll and morphological mutation induction in soybean (Glycine max (L.) Merrill.) through modified single seed bulk method. Acad. J. Agric. Res. 5(9): 218-223

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